Journal: Clinics (Sao Paulo, Brazil)

Article Title: Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca 2+ -CaM signaling pathway.

doi: 10.1016/j.clinsp.2024.100485

Figure Lengend Snippet: Fig. 2. Enhanced mAChRs expression in post-anal incision pain rat model. (A) Elevated Acetylcholine (Ach) Levels in the Model Group. (B) Increased Expression of M2 mAChR Subtype. (C) Increased Expression of M3 mAChR Subtype. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sham group.

Article Snippet: The membranes were blocked in 5% non-fat milk solution and subsequently probed with primary antibodies specific to IP3 (ab5804, Abcam, UK), CAM (MA3-917, Thermo Fisher, USA), M2 mAChR (sc33712, Santa Cruz, USA), M3 mAChR (sc-518107, Santa Cruz, USA), and beta-actin (A5316, Sigma-Aldrich, USA).

Techniques: Expressing

Journal: Clinics (Sao Paulo, Brazil)

Article Title: Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca 2+ -CaM signaling pathway.

doi: 10.1016/j.clinsp.2024.100485

Figure Lengend Snippet: Fig. 3. mAChRs knockout reduces anal pain sensitivity. (A) Diminished Pain Sensitivity in mAChRs Knockout Mice (SD group vs. mAChRs(-/-) group). (B) Western blot for M2 and M3 mAChR Expression. (C) Immunohistochemistry (IHC) for M2 and M3 mAChR Expression. (D) Positive Rate of M2 and M3 mAChRs. (E) Anal Sphincter Resting Pressure Measurements. (F) Number of Fecal Pellets Counted Postoperatively. *p < 0.05, **p < 0.01, ***p < 0.001 vs. SD group.

Article Snippet: The membranes were blocked in 5% non-fat milk solution and subsequently probed with primary antibodies specific to IP3 (ab5804, Abcam, UK), CAM (MA3-917, Thermo Fisher, USA), M2 mAChR (sc33712, Santa Cruz, USA), M3 mAChR (sc-518107, Santa Cruz, USA), and beta-actin (A5316, Sigma-Aldrich, USA).

Techniques: Knock-Out, Western Blot, Expressing, Immunohistochemistry

Journal: Clinics (Sao Paulo, Brazil)

Article Title: Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca 2+ -CaM signaling pathway.

doi: 10.1016/j.clinsp.2024.100485

Figure Lengend Snippet: Fig. 5. Electroacupuncture alleviates postoperative anal pain. (A) Reduced Pain Sensitivity (Vonfrey) following Electroacupuncture Treatment. (B) Altered Anal Sphincter Resting Pressure Measurements. (C) Improved Bowel Motility as Indicated by Number of Fecal Pellets. (D) Lowered Acetylcholine (Ach) Levels. (E) Western blot for M2 and M3 mAChR Expression. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group.

Article Snippet: The membranes were blocked in 5% non-fat milk solution and subsequently probed with primary antibodies specific to IP3 (ab5804, Abcam, UK), CAM (MA3-917, Thermo Fisher, USA), M2 mAChR (sc33712, Santa Cruz, USA), M3 mAChR (sc-518107, Santa Cruz, USA), and beta-actin (A5316, Sigma-Aldrich, USA).

Techniques: Western Blot, Expressing, Control